FluoProbes® 647H dye (FP-647H) : 653/675nm
- Standard far red filters (Cy5TM, APC) compatible
- Improved fluorescence and photostability rate
Apoptosis induction by Kp-10 on Jurkat cells and use of FP-647H in flow cytometry detection.
- GPR54 receptor (c-Myc tagged) staining with antibody clone 9E10 and Goat Anti Mouse conjugated with FP-647H or competitor (FL3 reading)
- Apoptosis staning with AnnexinV-FluoProbes® 488 (FL1 reading)
Apoptosis is inducted by GPR54 ligand: Kp-10.
After 16 hours, apoptotic cells (right-hand corner) have almost lost GPR54 expression though non-apoptotic cells still express the recptor.
FL3 = 633nm/ filter 660/20nm
