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LIFE SCIENCES > Immunodetection Tools > COVID-19 RESEARCH > RT-qPCR and RNA extraction kits

Covid-19: RT-qPCR and RNA extraction kits

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We offer a range of RT-qPCR kits for testing the new coronavirus strain COVID-19. Our molecular kits are specific in obtaining accurate results, specially in avoiding false-negative outcomes.  
   
   

Total RNA Purification Kit

    
For rapid purification of total RNA - including microRNA - without phenol.
  • Extract high quality & purity total RNA including miRNA
  • No phenol step required
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Efficiently extract small RNA irrespective of GC content
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Convenient & fast spin column format
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
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Ref.   Description   Packing
17200   Total RNA Purification Kit   50 preps
37500   Total RNA Purification Kit   100 preps
17250   Total RNA Purification Kit   250 preps
17270   Total RNA Purification Kit   500 preps
24350   Total RNA Purification 96-Well Kit (Deep Well Format, height 3.8 cm)   2 plates
24380   Total RNA Purification 96-Well Kit (Deep Well Format, height 3.8 cm)   6 plates
         
         

2019-nCoV TaqMan RT-PCR Kit

    
Norgen’s 2019-nCoV TaqMan RT-PCR Kit is designed for the detection of SARS-CoV-2 specific RNA in a real-time RT-PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of SARS-CoV-2 specific RNA is based on TaqMan one-step RT-PCR providing a simple, reliable and rapid result for the detection of SARS-CoV-2 infection. Norgen’s 2019-nCoV TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result.   RT-qPCR_Covid-19_coronavirus_research_Interchim01_0420
The 2019-nCoV TaqMan RT-PCR Kit comprises Master Mix for the target and PCR control detection, 4 target Primer & Probe Mixes, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagen.
     
     

Detection kit for SARS-CoV-2, which causes COVID-19 (2019-nCoV):

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Ready to use format containing everything required for detection Available in 1 format for analysis  
     
Ref.   Description   Packing
TM67100   2019-nCoV TaqMan RT-PCR Kit   50 reactions
TM67120   2019-nCoV TaqMan RT-PCR Kit   500 reactions
         
         

PCR Detection Solutions for Coronavirus

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For rapid purification of total RNA - including microRNA - without phenol.
  • COVID-19 detection based on the assays and protocols developed by the CDC
  • Primer & Probe Mixes contain all 3 CDC developed assays in individual tubes
  • All assays are premixed to the working concentrations recommended by the CDC
  • Positive Control contains 2 nCoV nucleocapsid target gene RNA (N1,N2) and RNase P (internal control) which are compatible with the CDC primer/probe set
  • Convenient ready-to-use 2X One-Step RT-PCR Master Mix
  • Manufactured under ISO 13485:2016 for research use only
     
Ref.   Description   Packing
TM67101   2019-nCoV Primer & Probe Mixes   50 reactions (per tube)
PC67102   2019-nCoV RT-PCR Positive Control   50 µL
TM67130   2019-nCoV Primer & Probe Mixes   500 reactions (per tube)
PC67120   2019-nCoV Positive Control   500 µL
28113   2X One-Step RT-PCR Master Mix   100 reactions
28115   2X One-Step RT-PCR Master Mix   500 reactions
         
         

Fast Plus Evagreen qPCR Master Mix

    
Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using the environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium´s PCR master mix delivers superior performance even with the most challenging samples tested.   Logo_Biotium_Interchim_0420
     
Covid-19_coronavirus_research_Interchim013_0420   EvaGreen® PCR dye is bright and sensitive. Comparison between an EvaGreen® qPCR Master Mix from Biotium and two fast SYBR® Green master mixes from two leading companies (company A and company Q) under similar condition. The inset is an enlarged view of the area near the baseline for better viewing the curve patterns of the weaker signals of the two SYBR®-based master mixes. Amplicon: ATPG fragment of human genomic DNA; instrument: ABI 7900 Fast.
     
The master mix is offered in three formats containing different amounts of ROX reference dye for use with different PCR instruments (without ROX, low ROX, and high ROX).
     
Ref.   Description   Packing
#31014-T   FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX   Trial size (1 mL)
#31014   FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX   2 x 1 mL
#31014-1   FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX   5 x 1 mL
#31014-2   FAST PLUS EVAGREEN qPCR MASTER MIX - Low ROX   50 x 1 mL
         
         

ABScript II One Step RT-qPCR Probe Kit (RK20407)

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ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates.

The one-step format significantly improves sensitivity and effectively prevent contamination.
The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5 ´-3 ´exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript II One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample.

Features :
    • From RNA sample to qPCR data only need one step;
    • The core reverse transcripase in this kit is ABclonal´s ABScript II, which could work in various situations. It has advantage of sensitivity and efficiency;
    • Suitable for multiplex PCR;
    • Could be applied in research, clinical /medical, industrial probe-based RT-qPCR reactions;
    • Compatible for all machine types, choosing the ROX types as you wish 
     

Result Using synthesed sequence from 2019-nCov
 

  
RT-qPCR_Covid-19_coronavirus_research_Interchim02_0420 Using the Pseudovirus, synthesed sequence from 2019-nCov, as the template, diluted into 105, 104, 103, 102 and 10 copies. Targets are ORF 1ab gene and N gene (Probes are recommended by CCDC).
The target could be well detected by ABclonal´s kit.
     
Ref.   Description   Packing
RK20407   ABScript II One Step RT-qPCR Probe Kit   50 reactions
RK20407   ABScript II One Step RT-qPCR Probe Kit   100 reactions


 		        

For more information, please see our Covid-19 related article (in french).

In progress. Due to the current situation, this page may be subject to frequent updates.
For any request please contact-us.
 
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INTERCHIM BIOSCIENCE RNA/DNA Isolation L6 Buffer - Ready to use
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P/N : BFVZG0     Pack : 1 x 150 ml  
P/N : BFVZG0         Item : BFVZG0         Pack : 1 x 150 ml  
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ABM GenomeCoV19 Detection Kit, CE-marked (for IVD) naso/oropharyngeal COVID-19 SARS-CoV-2 by RT-qPCR
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P/N : G628     Pack : 01 x 100 TS  
P/N : G628         Item : B8DRW0         Pack : 01 x 100 TS  
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BIO-HELIX COVID-19 MUTATION RT-QPCR DETECTION KIT (ALPHA, DELTA, KAPPA)
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P/N : QP019M-0050     Pack : 1 x 50 TS  
P/N : QP019M-0050         Item : BWNGX0         Pack : 1 x 50 TS  
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BIO-HELIX COVID-19 RT-QPCR DETECTION KIT PLUS (TFDA)
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P/N : QP019T-0100     Pack : 1 x 100 TS  
P/N : QP019T-0100         Item : BWNGW0         Pack : 1 x 100 TS  
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BIO-HELIX SARS-COV-2/COVID-19 RT-QPCR DETECTION KIT (RUO)
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P/N : QP019-0100     Pack : 1 x 100 TS  
P/N : QP019-0100         Item : B8DGN0         Pack : 1 x 100 TS  
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BIOMAXIMA GeneMATRIX Viral RNA/DNA Purification Kit, RNA/DNA longer than 200 nt from plasma, serum or other ce
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P/N : E3592     Pack : 01 x 100 TS  
P/N : E3592         Item : BFXIT0         Pack : 01 x 100 TS  
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BIOMAXIMA SARS-CoV-2 Real Time PCR LAB-KIT (CE mark, IVD), 8-well strips
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P/N : PCR5008     Pack : 01 x 96 TS  
P/N : PCR5008         Item : B8DHQ0         Pack : 01 x 96 TS  
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BIOMAXIMA SARS-CoV-2 Real Time PCR LAB-KIT (CE mark, IVD), 96-well plate
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P/N : PCR5096     Pack : 01 x 96 TS  
P/N : PCR5096         Item : B8DHR0         Pack : 01 x 96 TS  
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ELISABETH PHARMACON EliGene COVID19 BASIC A RT kit, detection of SARSCoV-2 viral RNA by one-step RT-qPCR assay, CE-IVD
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P/N : 90077-RT-A500     Pack : 01 x 500 TS  
P/N : 90077-RT-A500         Item : BH4MT1         Pack : 01 x 500 TS  
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ELISABETH PHARMACON EliGene COVID19 BASIC A RT kit, detection of SARSCoV-2 viral RNA by one-step RT-qPCR assay, CE-IVD
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P/N : 90077-RT-A     Pack : 01 x 100 TS  
P/N : 90077-RT-A         Item : BH4MT0         Pack : 01 x 100 TS  
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ELISABETH PHARMACON EliGene COVID19 CONFIRM RT for the detection or confirmation of SARSCoV-2 viral RNA, CE-IVD
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P/N : 90078-RT     Pack : 01 x 100 TS  
P/N : 90078-RT         Item : BH4MU0         Pack : 01 x 100 TS  
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ELISABETH PHARMACON EliGene COVID19 CONFIRM RT for the detection or confirmation of SARSCoV-2 viral RNA, CE-IVD
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P/N : 90078-RT-500     Pack : 01 x 500 TS  
P/N : 90078-RT-500         Item : BH4MU1         Pack : 01 x 500 TS  
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ELISABETH PHARMACON EliGene COVID19 Triple RIC RT for SARS-CoV-2,H1N1,H3N2,H5N1,Influenza B virus,RSV A and B (CE-IVD)
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P/N : 90079-RT-500     Pack : 01 x 500 TS  
P/N : 90079-RT-500         Item : BH4MW1         Pack : 01 x 500 TS  
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ELISABETH PHARMACON EliGene COVID19 Triple RIC RT for SARS-CoV-2,H1N1,H3N2,H5N1,Influenza B virus,RSV A and B (CE-IVD)
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P/N : 90079-RT     Pack : 01 x 100 TS  
P/N : 90079-RT         Item : BH4MW0         Pack : 01 x 100 TS  
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ELISABETH PHARMACON EliGene Viral DNA/RNA Isolation Kit, CE-IVD mark
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P/N : 409100     Pack : 01 x 100 TS  
P/N : 409100         Item : BH4MX0         Pack : 01 x 100 TS  
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ELISABETH PHARMACON EliGene Viral RNA/DNA FAST 96 Vacuum Isolation Kit, CE-IVD mark
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P/N : 409480     Pack : 01 x 480 TS  
P/N : 409480         Item : BH4MY0         Pack : 01 x 480 TS  
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ELISABETH PHARMACON ZEPHYRUS Vacuum Pump Manifold System for processing of 24 mini, 8 midi or maxi columns, 96-well plat
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P/N : ZVPMS-300     Pack : 01 x 1 U.  
P/N : ZVPMS-300         Item : BH4N00         Pack : 01 x 1 U.  
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FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT I, for low viral load specimen using carrier RNA
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P/N : FAVNK001     Pack : 1 x 50 TS  
P/N : FAVNK001         Item : EMV800         Pack : 1 x 50 TS  
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FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT I, for low viral load specimen using carrier RNA
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P/N : FAVNK001-1     Pack : 1 x 100 TS  
P/N : FAVNK001-1         Item : EMV801         Pack : 1 x 100 TS  
Cat. Price :
 
 
FAVORGEN BIOTECH VIRAL NUCLEIC ACID EXTRACTION KIT I, for low viral load specimen using carrier RNA
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P/N : FAVNK001-2     Pack : 1 x 300 TS  
P/N : FAVNK001-2         Item : EMV802         Pack : 1 x 300 TS  
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JENA BIOSCIENCE SWAB DNA PREPARATION - COLUMN KIT, DNA PURIFICATION (E.G FROM BUCCAL AND SURFACES) FROM SWABS WITH S
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P/N : PP-238S     Pack : 1 x 50 TS  
P/N : PP-238S         Item : BVCMG0         Pack : 1 x 50 TS  
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NANOHELIX DirectFast SARS-CoV-2 multiplex assay
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P/N : DFSCV100     Pack : 01 x 100 TS  
P/N : DFSCV100         Item : BY0QAA         Pack : 01 x 100 TS  
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NORGEN BIOTEK 2019-nCoV TaqMan RT-PCR Kit, Detection kit for SARS-CoV-2, which causes COVID-19, CE-IVD marked
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P/N : DXTM67100     Pack : 1 x 50 TS  
P/N : DXTM67100         Item : BFWX90         Pack : 1 x 50 TS  
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NORGEN BIOTEK 2019-nCoV TaqMan RT-PCR Kit, Detection kit for SARS-CoV-2, which causes COVID-19, CE-IVD marked
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P/N : DXTM67120     Pack : 1 x 500 TS  
P/N : DXTM67120         Item : BFWX91         Pack : 1 x 500 TS  
Cat. Price :
 
 
NORGEN BIOTEK 2019-nCoV TaqMan RT-PCR Kit, Detection kit for SARS-CoV-2, which causes COVID-19
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P/N : TM67120     Pack : 1 x 500 TS  
P/N : TM67120         Item : B8D9IB         Pack : 1 x 500 TS  
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