Wide Pore Silica

Uptisphere^® Protein A HPLC columns combine the pressure benefits associated with HPLC and a silica based stationary phase with the affinity binding characteristics of Protein A. These columns can generally run on HPLC apparatus up to flow rates of 720cm /hr compared to classical Protein A agarose based affinity columns that work up to flow rates of 50 - to 60cm /hr.

Uptisphere^® Protein A bonded silica is generally used for Immunglobulin purification
from research to process scale.

• No issues of swelling
• High mechanical stability
• Thermal stability
• Compatible with water and organic solvents
• High capacity at high flow velocities: 45mg/ml
• Protein recovery: 98 - 100%