HALO by AMT
Application n°123-DA
![Image](http://www.interchim.fr/forum_img/chromato/AMT_123-DA_Figure1.jpg)
TEST CONDITIONS:
Column: 2.1 x 100 mm, HALO 2.7 m Penta-HILIC
Part Number: 92812-605
Mobile Phase A: 5 mM Ammonium Formate, pH 3
Mobile Phase B: Acetonitrile
Isocratic: premixed 5/95 A/B
Flow Rate: 0.5 mL/min.
Pressure: 149 bar
Temperature: 60°C
Detection: Selected Ion Monitoring as indicated
Injection Volume: 1 μL
Sample Solvent: 90/10 ACN/water
MS parameters: Positive ion mode, 2 kV, 400°C heat block 225°C capillary
LC-MS System: Shimadzu Nexera and LCMS-2020 (single quadrupole MS)
This mixture of drugs of abuse and metabolites is quickly identified using a HALO Penta-HILIC column and selected ion monitoring (SIM) for improved sensitivity. Adapted from J. Pharm. Anal. 2013; 3 (5): 303-311.
![Image](http://www.interchim.fr/forum_img/application_note_interchim.png)