HALO by AMT
Application n°119-B
![Image](http://www.interchim.fr/forum_img/chromato/AMT_119-B_Figure1.jpg)
TEST CONDITIONS:
Column: 2.1 x 100 mm, HALO 2 Penta-HILIC
Part Number: 91812-605
Isocratic: 92/8:ACN/water with 5 mM
Ammonium Formate buffer, pH 3
Flow Rate: 0.5 mL/min.
Pressure: 229 bar
Temperature: 30°C
Detection: UV 245 nm, PDA
Injection Volume: 1 μL
Sample Solvent: 90/10 ACN/0.1 M
Ammonium Formate buffer, pH 3
Data Rate: 40 Hz
Response Time: 0.1 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Agilent 1200 SL
![Image](http://www.interchim.fr/forum_img/chromato/AMT_119-B_Structures.jpg)
HALO 2 Penta-HILIC separates these five local anesthetics with good peak shape in less than 1.5 minutes.
![Image](http://www.interchim.fr/forum_img/application_note_interchim.png)