HALO by AMT
Application n°120-F
![Image](http://www.interchim.fr/forum_img/chromato/AMT_120-F_Figure1.jpg)
TEST CONDITIONS:
Column: 2.1 x 100 mm, HALO 2 HILIC
Part Number: 91812-601
Isocratic: 92/8: ACN/water with 5 mM
Ammonium Formate, pH 3
Flow Rate: 0.5 mL/min.
Pressure: 220 bar
Temperature: 30°C
Detection: UV 265 nm, PDA
Injection Volume: 0.3 μL
Sample Solvent: 75/25: ACN/Methanol with
2% formic acid
Data Rate: 40 Hz
Response Time: 0.1 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Agilent 1200 SL
![Image](http://www.interchim.fr/forum_img/chromato/AMT_120-F_Structures.jpg)
A fast separation of water soluble vitamins is accomplished with a HALO 2 HILIC column.
![Image](http://www.interchim.fr/forum_img/application_note_interchim.png)