The Miniblotter™ instruments enable you to perform multiple antibody or probe incubations on a single membrane, using microliter volumes of reagent. Typical applications include monoclonal antibody screening on western blots, animal and human serum screening, and DNA probe hybridizations. Sample processing is streamlined through use of the Washing Manifold, which enables washing of all sample lanes simultaneously. Reactions can be detected by all conventional methods, including enzyme conjugates, chemiluminescence and radiolabels.
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- For membrane assays including Western, Northern, and Southern Blots and Checker-board Blots
- Requires microliter volumes, not milliliter volumes
- Multiple samples analyzed simultaneously
- No cross-contamination between channels
- Decrease processing time
- All results on a single membrane, non need to strip
- Compatible with all standard electrophoresis systems
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Product Cat# |
Cat# (with washing manifold) |
Chanels/samples per blot |
Max rgt vol. |
Dimensions
channels |
Blots
dimensions |
Manifold |
Divers |
Miniblotter 45 Unit (for regular gel processing) |
987900 |
538370 |
45 channels |
110µL |
1.5 mm x 13 cm |
13 cm |
F2 |
(a) |
Miniblotter 25 Unit (for regular gel processing) |
BN4550 |
661060 |
25 channels |
250µL |
4.0 mm x 13 cm |
13 cm |
F2 |
- |
Miniblotter 28 - Single Unit (for mini gel processing) |
S31580 |
590550 |
28 channels |
50µL |
1.5 mm x 5.5 cm |
8.3 cm |
F1 |
(b) |
Miniblotter 28 - Dual Unit (for mini gel processing) |
168830 |
649400 |
2 x 28 channels |
50µL |
1.5 mm x 5.5 cm |
8.3 cm |
F1 |
(b) |
Miniblotter 20 - Single Unit (for mini gel processing) |
BN4580 |
572090 |
20 channels |
350µL |
3.0 mm x 5.5 cm |
8.3 cm |
F1 |
- |
Miniblotter 16 - Dual Unit |
S31580 |
590550 |
2 x 16 channels |
350µL |
3.0 mm x 5.5 cm |
8.3 cm |
F1 |
(c) |
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(a) Combs available for gel electrophoresis to line up Miniblotter channels with electrophoresis lane
(b) Ideal for hybridoma screening from 96-well microplates. Hybridoma supernatants can be transferred directly into Miniblotter 28 channels using an 8-channel automatic pipette.
(c) Similar to Miniblotter 20 - Single Unit, except that there are two sets of 16 channels so that two membrane blots can be placed between the Top and Bottom Plates allowing for the processing of up to 32 samples (16x2). |
Disposable Miniblot Systems for Minigel Processing
The Disposable Miniblot System operates exactly as the Miniblotter 28 unit, but its Top Plate frame (penned ´C-Shell´) holds a disposable cassette. In addition to a test-specific blot, this disposable plastic cassette offers the same sample ports and 28 (enclosed) reaction channels as the Miniblotter 28 Unit. In the Disposable Miniblot System, however, once the assay is completed and the blot has been developed, the disposable cassette is thrown away - minimizing operator contact with potentially harmful reagents.
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- Cassettes prevent risk of carry-over from one assay to another.
- Cassettes eliminate possibility of cross-contamination between samples
- Cassettes safely contain potentially hazardous serum during assay
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Cassette Miniblot - Single Unit |
Cat# (dual unit) |
Chanels/samples per blot |
Max rgt vol. |
Dimensions
channels |
Blots
dimensions |
Manifold |
Divers |
Cassette Miniblot - Single Unit |
982720 |
- |
28 channels |
50µL |
1.5 mm x 6 cm |
8.3 cm |
F1 |
(b) |
Cassette Miniblot - Dual Unit |
982721 |
- |
2 x 28 channels |
50µL |
1.5 mm x 6 cm |
8.3 cm |
F1 |
(b) |
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Please inquire for other blotting accessories (rapid downward transfer system Turboblotter #BN478, standard incubation trays #BN480, sealable hybridization folders #BN481,..). |
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